Quantitative evaluation of mouse peritoneal macrophage spreading by image analysis determination of the nucleo-cytoplasmic ratio
Damien Schovaert-Brossault,
Laboratoire d'Histologie-Embryologie-Cytogénétique (Pr. G. DAVID) CHU BICETRE, 78 rue du Gal Leclerc, 94270 Kremlin Bicetre, France
Monique Rocharveiller,
Laboratoire de Pharmacologie, CHU COCHIN, 24 rue du Fg St Jacques,75014 Paris, France
David Kaplan,
Stanford University, California, USA
Paule Michel,
Laboratoire d'Histologie-Embryologie-Cytogénétique, (Pr. B. SCHRAMM) CHU COCHIN, 24 rue du Fg St Jacques, 75014 Paris, France
Bertrand Mace,
Laboratoire d'Histologie-Embryologie-Cytogénétique, (Pr. B. SCHRAMM) CHU COCHIN, 24 rue du Fg St Jacques, 75014 Paris, France
Jean-Paul Giroud,
Laboratoire de Pharmacologie, CHU COCHIN, 24 rue du Fg St Jacques,75014 Paris, France
Alain Pompidou,
Laboratoire d'Histologie-Embryologie-Cytogénétique, (Pr. B. SCHRAMM) CHU COCHIN, 24 rue du Fg St Jacques, 75014 Paris, France; correspondence and reprints: Pompidou A.
Abstract
A method is proposed for measuring the nucleo-cytoplasmic ratio automatically. The examined samples are macrophages of mice obtained from the peritoneal cavity. A computer program has been devised for the measurement of the spreading of these macrophages using a Quantimet 720 analyser. Comparison with results obtained manually allowed us to demonstrate the reliability of the automatic analysis.
Para citar este artículo
Damien Schovaert-Brossault, Monique Rocharveiller, David Kaplan, Paule Michel, Bertrand Mace, Jean-Paul Giroud & Alain Pompidou, «Quantitative evaluation of mouse peritoneal macrophage spreading by image analysis determination of the nucleo-cytoplasmic ratio», Acta Stereologica [En ligne], Volume 2 (1983), Number 1 - Apr. 1983, 159-166 URL : https://popups.uliege.be/0351-580x/index.php?id=4138.