Acta Stereologica

0351-580X

 

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Denis Pichon, Hervé Vivier, Marie Noelle Pons, Anissa Lounes & Ahmed Lebrihi

Characterization and growth monitoring of filamentous microorganisms by image analysis

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Abstract

The growth of filamentous antibiotic productive bacteria Streptomyces ambofaciens in submerged culture is monitored via analysis of light microscopy images. The method provides data on the size of the microorganisms and classifies their morphological types into three populations: single filaments, entangled filaments and pellets. Two different growth media are studied, the first one contains valin which is a precursor of the antibiotic (spiramycin) and the second contains an ammonium salt as nitrogen source. Both kinetics of the distribution in number and in projected area of the morphological types during the fermentation are obtained.

Keywords : antibiotic, filamentous microorganisms, growth monitoring, image analysis

To cite this article

Denis Pichon, Hervé Vivier, Marie Noelle Pons, Anissa Lounes & Ahmed Lebrihi, «Characterization and growth monitoring of filamentous microorganisms by image analysis», Acta Stereologica [En ligne], Volume 13 (1994), Number 1 - Proceedings of the sixth European congress for stereology - Part two - May 1994, 215-220 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4628.

About: Denis Pichon

Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-INPL, BP 451, 54000 F-Nancy cedex, France; Procell S.A. 8, Allée Pelletier Doisy, 54600 F-Villers les Nancy, France

About: Hervé Vivier

Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-INPL, BP 451, 54000 F-Nancy cedex, France

About: Marie Noelle Pons

Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-INPL, BP 451, 54000 F-Nancy cedex, France

About: Anissa Lounes

Laboratoire de Microbiologie Industrielle, ENSAIA-INPL 2, Av. Forêt de Haye, 54500 F-Vandoeuvre les Nancy, France

About: Ahmed Lebrihi

Laboratoire de Microbiologie Industrielle, ENSAIA-INPL 2, Av. Forêt de Haye, 54500 F-Vandoeuvre les Nancy, France