Acta Stereologica

0351-580X

 

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Marjo W. Kapraali, Olle Johansson & Andrés Uribe

Stereological estimation of the total volume of duodenal mucosa and of endocrine cells in rats treated with prostaglandin E2

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Abstract

Stereological methods were applied on histological sections of 2 cm rat duodenum to determine the total volume of the mucosa, the total surface area of small intestinal epithelial lining and the total volume of endocrine cells. The effects of different oral doses of prostaglandin E2 and a methyl analogue on the parameters above were estimated following 4 weeks of treatment. The total volume of serotonin-immunoreactive cells was increased by prostaglandins E2, whereas the analogue reduced the total volume of somatostatin-immunoreactive cells compared to controls (p<0.05). The total mucosal volume and the total surface area of small intestinal epithelial lining were unaffected. Despite overestimation of endocrine cells in thick sections, the estimation of the profile area by image analysis disclosed that the changes in total volume induced by prostaglandins was most likely to be secondary to hyperplasia of endocine cells.

Keywords : enterochromaffin cell, prostaglandin E2, small intestine, somatostatin, stereology

To cite this article

Marjo W. Kapraali, Olle Johansson & Andrés Uribe, «Stereological estimation of the total volume of duodenal mucosa and of endocrine cells in rats treated with prostaglandin E2», Acta Stereologica [En ligne], Volume 13 (1994), Number 1 - Proceedings of the sixth European congress for stereology - Part two - May 1994, 27-30 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4574.

About: Marjo W. Kapraali

Department of Medicine, Karolinska Institute, Danderyd Hospital, S-18288 Danderyd; Department of Medicine, University Hospital, Uppsala, Sweden

About: Olle Johansson

Department of Histology and Neurobiology, Karolinska Institute, Stockholm, Sweden

About: Andrés Uribe

Department of Medicine, Karolinska Institute, Danderyd Hospital, S-18288 Danderyd; Department of Medicine, University Hospital, Uppsala, Sweden