Acta Stereologica Acta Stereologica -  Volume 2 (1983)  Number 2 - Proceedings of the second symposium on morphometry in morphological diagnoses, september 7-9, 1983, Kuopio, Finland - Dec. 1983 

Morphometry of kidney biopsy: number of glomeruli and accuracy of morphometric measurements

Yrjö Collan
Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland
Esa Jantunen
Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland
Jouko Karhunen
Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland
Timo Romppanen
Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland

Abstract

Five kidney biopsies with diffuse changes containing 16 - 48 glomeruli were analysed morphometrically. After excluding profiles with a diameter smaller than 60% of the diameter of the largest glomerular tuft in the biopsy the coefficient of variation between glomeruli was 12 - 19 per cent for the following parameters: number of nuclei per glomerular area, surface density of glomerular BM, and the volume density of capillaries. The volume density of mesangium showed a CV of 27%. The biopsy should contain 1 - 4 glomerular profiles if one would like to keep the relative standard error (CV/√n) of these parameters under 15%. The estimates were tested by letting a computer to sample a glomerular data file in samples of 1 - 10 glomeruli. The probabilities for deviations larger than 10 - 20% of the unbiased value were calculated. Cellular proliferation (nuclear density) in the glomeruli can be estimated in biopsies with 2 glomeruli with a probability of 12% for a deviation of more than 20%. In samples with 6 glomeruli no nuclear density figures deviated more than 20% of the unbiased estimate.

To cite this article

Yrjö Collan, Esa Jantunen, Jouko Karhunen & Timo Romppanen, «Morphometry of kidney biopsy: number of glomeruli and accuracy of morphometric measurements», Acta Stereologica [En ligne], Volume 2 (1983), Number 2 - Proceedings of the second symposium on morphometry in morphological diagnoses, september 7-9, 1983, Kuopio, Finland - Dec. 1983, 380 - 383 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4311.