Acta Stereologica

0351-580X

 

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Yrjö Collan, Esa Jantunen, Jouko Karhunen & Timo Romppanen

Morphometry of kidney biopsy: number of glomeruli and accuracy of morphometric measurements

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Abstract

Five kidney biopsies with diffuse changes containing 16 - 48 glomeruli were analysed morphometrically. After excluding profiles with a diameter smaller than 60% of the diameter of the largest glomerular tuft in the biopsy the coefficient of variation between glomeruli was 12 - 19 per cent for the following parameters: number of nuclei per glomerular area, surface density of glomerular BM, and the volume density of capillaries. The volume density of mesangium showed a CV of 27%. The biopsy should contain 1 - 4 glomerular profiles if one would like to keep the relative standard error (CV/√n) of these parameters under 15%. The estimates were tested by letting a computer to sample a glomerular data file in samples of 1 - 10 glomeruli. The probabilities for deviations larger than 10 - 20% of the unbiased value were calculated. Cellular proliferation (nuclear density) in the glomeruli can be estimated in biopsies with 2 glomeruli with a probability of 12% for a deviation of more than 20%. In samples with 6 glomeruli no nuclear density figures deviated more than 20% of the unbiased estimate.

To cite this article

Yrjö Collan, Esa Jantunen, Jouko Karhunen & Timo Romppanen, «Morphometry of kidney biopsy: number of glomeruli and accuracy of morphometric measurements», Acta Stereologica [En ligne], Volume 2 (1983), Number 2 - Proceedings of the second symposium on morphometry in morphological diagnoses, september 7-9, 1983, Kuopio, Finland - Dec. 1983, 380 - 383 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4311.

About: Yrjö Collan

Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland

About: Esa Jantunen

Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland

About: Jouko Karhunen

Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland

About: Timo Romppanen

Department of Pathology, University of Kuopio, P.O.B. 6, 70211 Kuopio 21, Finland