Acta Stereologica

0351-580X

 

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Damien Schovaert-Brossault, Monique Rocharveiller, David Kaplan, Paule Michel, Bertrand Mace, Jean-Paul Giroud & Alain Pompidou

Quantitative evaluation of mouse peritoneal macrophage spreading by image analysis determination of the nucleo-cytoplasmic ratio

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Abstract

A method is proposed for measuring the nucleo-cytoplasmic ratio automatically. The examined samples are macrophages of mice obtained from the peritoneal cavity. A computer program has been devised for the measurement of the spreading of these macrophages using a Quantimet 720 analyser. Comparison with results obtained manually allowed us to demonstrate the reliability of the automatic analysis.

Om dit artikel te citeren:

Damien Schovaert-Brossault, Monique Rocharveiller, David Kaplan, Paule Michel, Bertrand Mace, Jean-Paul Giroud & Alain Pompidou, «Quantitative evaluation of mouse peritoneal macrophage spreading by image analysis determination of the nucleo-cytoplasmic ratio», Acta Stereologica [En ligne], Volume 2 (1983), Number 1 - Apr. 1983, 159-166 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=4138.

Over : Damien Schovaert-Brossault

Laboratoire d'Histologie-Embryologie-Cytogénétique (Pr. G. DAVID) CHU BICETRE, 78 rue du Gal Leclerc, 94270 Kremlin Bicetre, France

Over : Monique Rocharveiller

Laboratoire de Pharmacologie, CHU COCHIN, 24 rue du Fg St Jacques,75014 Paris, France

Over : David Kaplan

Stanford University, California, USA

Over : Paule Michel

Laboratoire d'Histologie-Embryologie-Cytogénétique, (Pr. B. SCHRAMM) CHU COCHIN, 24 rue du Fg St Jacques, 75014 Paris, France

Over : Bertrand Mace

Laboratoire d'Histologie-Embryologie-Cytogénétique, (Pr. B. SCHRAMM) CHU COCHIN, 24 rue du Fg St Jacques, 75014 Paris, France

Over : Jean-Paul Giroud

Laboratoire de Pharmacologie, CHU COCHIN, 24 rue du Fg St Jacques,75014 Paris, France

Over : Alain Pompidou

Laboratoire d'Histologie-Embryologie-Cytogénétique, (Pr. B. SCHRAMM) CHU COCHIN, 24 rue du Fg St Jacques, 75014 Paris, France; correspondence and reprints: Pompidou A.