Acta Stereologica Acta Stereologica -  Volume 3 (1984)  Number 1 - May 1984 

Reproducibility in morphometric assessment of positive staining for CEA in ovarian tumours

Veli-Matti Kosma
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Yrjö Collan
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Marja-Leena Aalto
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Anders Seppä
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Markus Rautiainen
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Kalevi Selkäinaho
Department of Applied Mathematics, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland

Abstract

Positive staining for carcinoembryonic antigen (CEA) in 30 ovarian tumours was assessed subjectively and morphometrically. The kappa coefficient (κ) and the intraclass correlation coefficient (ICC) were used to assess reproducibility. On subjective grading by 5 observers κ was 0.51 (90% confidence limits: 0.39-0.63) and by one observer (5 estimates) 0.96 (0.92-1). On morphometry the ICC of individual fields (5-person test) was 0.49-0.81 (0.26-0.93) and of standardized fields 0.64-0.78 (0.28-0.96), respectively. The ICC in 12 samples (5-person test, interobserver reproducibility) was 0.78-0.93 (0.31-0.99). In an intraobserver test (5 measurements) ICC was 0.87-0.95 (0.45-0.98). The results suggest that morphometric assessment is more reproducible than subjective assessment and that the human variations in counting account for most of the variation in morphometric reproducibility.

To cite this article

Veli-Matti Kosma, Yrjö Collan, Marja-Leena Aalto, Anders Seppä, Markus Rautiainen & Kalevi Selkäinaho, «Reproducibility in morphometric assessment of positive staining for CEA in ovarian tumours», Acta Stereologica [En ligne], Volume 3 (1984), Number 1 - May 1984, 71-74 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3956.