Acta Stereologica Acta Stereologica -  Volume 3 (1984)  Number 1 - May 1984 

Automatic detection of sphere distribution in thick section

Kensuke Baba
Department of Pathology and Surgical Pathology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807, Japan
Kiyoshi Miyamoto
Institute of Medical Sciences, Dokkyo University School of Medicine, Japan
Kazumonto Kimura
Institute of Medical Sciences, Dokkyo University School of Medicine, Japan
Hidetaka Matsukuma
Department of Pathology and Surgical Pathology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807, Japan
Osamu Koide
Department of Pathology and Surgical Pathology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807, Japan

Abstract

The size distribution of somatotroph secretory granules on electronmicrographes of rat adenohypophysis is automatically detected by two methods; 1) "erosion-chord method" and 2) "distance-maximum method". In the later method, the diameter of each disc of the granules is automatically detected in a way which depends upon Rosenfeld's distance function. In the former method, the Serra's erosion is employed prior to the detection of the chord length to separate the profile of the granules on the electron micrographs. The two results are compared with the results using the manual methods. In these analyses, the thickness of the section is stereologically introduced.

To cite this article

Kensuke Baba, Kiyoshi Miyamoto, Kazumonto Kimura, Hidetaka Matsukuma & Osamu Koide, «Automatic detection of sphere distribution in thick section», Acta Stereologica [En ligne], Volume 3 (1984), Number 1 - May 1984, 57-62 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3945.