Acta Stereologica

0351-580X

 

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Fabio Benfenati, Mauro Cimino, Kjell Fuxe & Luigi F. Agnati

Computer assisted image analysis of transmitter receptors

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Abstract

Quantitative methodologies for the analysis of autoradiograms have been developed using computerized image analyzers coupled to standard TV camera input for microdensitometrical evaluations. The procedures include the assessment of the film response to radioactivity using appropriate standards calibrated according to brain tissue quenching (grey matter) and a non-linear conversion of optical density measurements into radioactivity values. In this procedure the best fitting mathematical model has been adopted to give as low variability as possible in the transformation of the data. The reliability of this approach has been evaluated both by means of computer assisted Monte-Carlo simulation procedures and by parallel biochemical determinations of the binding characteristics of transmitter receptors.

Keywords : brain tissue quenching, film response, Monte-Carlo simulation procedures, receptor autoradiography

Para citar este artículo

Fabio Benfenati, Mauro Cimino, Kjell Fuxe & Luigi F. Agnati, «Computer assisted image analysis of transmitter receptors», Acta Stereologica [En ligne], Volume 4 (1985), Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985, 193-198 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3170.

Acerca de: Fabio Benfenati

Department of Human Physiology, University of Modena, Modena, Italy

Acerca de: Mauro Cimino

Department of Pharmacology, University of Urbino, Italy

Acerca de: Kjell Fuxe

Department of Histology, Karolinska Institutet, Stockholm, Sweden

Acerca de: Luigi F. Agnati

Department of Human Physiology, University of Modena, Modena, Italy