Acta Stereologica Acta Stereologica -  Volume 4 (1985)  Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985 

Image analysis and the determination of the entity of coexistence of neuroactive substances in nerve cell body populations

Luigi F. Agnati
Department of Human Physiology, University of Modena, Modena, Italy
Kjell Fuxe
Department of Histology, Karolinska Institutet, Stockholm, Sweden
Ann Marie Janson
Department of Histology, Karolinska Institutet, Stockholm, Sweden
R. Grimaldi
Department of Human Physiology, University of Modena, Modena, Italy
Isabella Zini
Department of Human Physiology, University of Modena, Modena, Italy
Anders Härfstrand
Department of Histology, Karolinska Institutet, Stockholm, Sweden

Abstract

Automatic image analysis has been employed to use the occlusion method for the quantitative determination of coexistence. Also a method has been developed using the dilatation function of the IBAS image analyzer combined with the performance of a logical operation “and”, to quantitatively evaluate the codistribution of profiles. Thus, this procedure allowed the determination of a dilated overlap area (FADAB), which could be related to the mean dilated field area (FADA +FADB)/2.

Keywords : codistribution, coexistence, image analysis, neurons, transmitter

To cite this article

Luigi F. Agnati, Kjell Fuxe, Ann Marie Janson, R. Grimaldi, Isabella Zini & Anders Härfstrand, «Image analysis and the determination of the entity of coexistence of neuroactive substances in nerve cell body populations», Acta Stereologica [En ligne], Volume 4 (1985), Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985, 175-180 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3146.