Acta Stereologica

0351-580X

 

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Luigi F. Agnati, Kjell Fuxe, Ann Marie Janson, R. Grimaldi, Isabella Zini & Anders Härfstrand

Image analysis and the determination of the entity of coexistence of neuroactive substances in nerve cell body populations

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Abstract

Automatic image analysis has been employed to use the occlusion method for the quantitative determination of coexistence. Also a method has been developed using the dilatation function of the IBAS image analyzer combined with the performance of a logical operation “and”, to quantitatively evaluate the codistribution of profiles. Thus, this procedure allowed the determination of a dilated overlap area (FADAB), which could be related to the mean dilated field area (FADA +FADB)/2.

Keywords : codistribution, coexistence, image analysis, neurons, transmitter

To cite this article

Luigi F. Agnati, Kjell Fuxe, Ann Marie Janson, R. Grimaldi, Isabella Zini & Anders Härfstrand, «Image analysis and the determination of the entity of coexistence of neuroactive substances in nerve cell body populations», Acta Stereologica [En ligne], Volume 4 (1985), Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985, 175-180 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3146.

About: Luigi F. Agnati

Department of Human Physiology, University of Modena, Modena, Italy

About: Kjell Fuxe

Department of Histology, Karolinska Institutet, Stockholm, Sweden

About: Ann Marie Janson

Department of Histology, Karolinska Institutet, Stockholm, Sweden

About: R. Grimaldi

Department of Human Physiology, University of Modena, Modena, Italy

About: Isabella Zini

Department of Human Physiology, University of Modena, Modena, Italy

About: Anders Härfstrand

Department of Histology, Karolinska Institutet, Stockholm, Sweden