Acta Stereologica

0351-580X

 

since 05 December 2013 :
View(s): 337 (3 ULiège)
Download(s): 65 (1 ULiège)
print        
Michel Kraemer, Jeannie Vassy, Marie-Thérèse Chalumeau & Albrecht Reith

Ultrastructural and morphometric investigation on the fetal rat liver

(Volume 4 (1985) — Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985)
Article
Open Access

Attached document(s)

Annexes

Abstract

Qualitative and quantitative changes in liver tissue composition have been studied during prenatal development of the rat by electron microscopy and morphometric/stereologic methods. On the 12th day of gestation 26% of fetal liver volume consisted of hepatocytes, and the non hepatocytes cells amounted to 57%. On the 13th and 18th fetal days the volumetric density of hepatocytes occupied about 40% of the liver, and the non hepatocyte cells amounted respectively to 50% and 43%. By days 12 and 13, the rough endoplasmic reticulum and the Golgi complexes were well differentiated indicating that young fetal hepatocytes were able to synthesize and export plasma proteins. On days 12, 13 and 18 the mitochondrial compartment occupied 13% of the hepatocyte cytoplasm. On day 18, the mitochondria were larger and less numerous than on days 12 and 13, but without change in the shape. The ferrocyanide-reduced osmium post-fixation has been compared with conventional osmium post-fixation for the 18 days old fetuses. Ferrocyanide-reduced osmium post-fixation combined a good preservation and staining of glycogen with an increase density of membrane organelles.

Keywords : development, electron microscopy, hepatocytes, morphometry, rat, stereology

To cite this article

Michel Kraemer, Jeannie Vassy, Marie-Thérèse Chalumeau & Albrecht Reith, «Ultrastructural and morphometric investigation on the fetal rat liver», Acta Stereologica [En ligne], Volume 4 (1985), Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985, 127-132 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3089.

About: Michel Kraemer

Université Paris XIII, UER Biomédicale, Laboratoire de Biologie, 74 rue Marcel Cachin, 93012 Bobigny cedex, France

About: Jeannie Vassy

Université Paris XIII, UER Biomédicale, Laboratoire de Biologie, 74 rue Marcel Cachin, 93012 Bobigny cedex, France

About: Marie-Thérèse Chalumeau

Université Paris XIII, UER Biomédicale, Laboratoire de Biologie, 74 rue Marcel Cachin, 93012 Bobigny cedex, France

About: Albrecht Reith

Det Norske Radiumhospital, Laboratory for Electron Microscopy and Morphometry, Montebello, Oslo 3, Norway