Acta Stereologica

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Miroslav Kališnik & Zdenka Pajer

Simplified differential counting of particles in light microscopy

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Abstract

A simplification of the differential counting of particles can be obtained by double counting the particles in the same slice: firstly, through the whole slice thickness and secondly, in the optical section only. This method was empirically tested on the epithelial nuclei of the adult male mouse, namely on five step slices of one thyroid lobe. It showed that the results obtained by simplified differential counting and the original one were of the same class size. The economy index was higher with the modified method. But, the economy of the simplified method is still greater because there is no need to prepare two differently thick section series. On this basis the simplified differential counting of particles in light microscopy can be recommended as an alternative method with higher economy.

Keywords : depth of focus, differential particle counting, light microscopy, numerical density, slice thickness, thyroid gland

To cite this article

Miroslav Kališnik & Zdenka Pajer, «Simplified differential counting of particles in light microscopy», Acta Stereologica [En ligne], Volume 4 (1985), Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985, 121-126 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=3070.

About: Miroslav Kališnik

Institute of Histology and Embryology, Medical Faculty, University E. Kardelj, Zaloška 4/I, P.O.Box 10, 61105 Ljubljana, Yugoslavia

About: Zdenka Pajer

Institute of Histology and Embryology, Medical Faculty, University E. Kardelj, Zaloška 4/I, P.O.Box 10, 61105 Ljubljana, Yugoslavia