Acta Stereologica

0351-580X

 

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Verica Milošević, Mirjana Hristić, Dobrila Kalafatić, Vesna Koko & Gordana Ušćebrka

Stereological analysis of the adrenal gland cortex in the male rats treated with hydrocortisone

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Abstract

In this work, the reaction of the adrenal cortex of the male rats to treatment with different hydrocortisone doses applied during the juvenile stage of development was studied using stereological methods. Animals were divided into 6 groups. Groups 1, 2, 3 and 5 were treated for four weeks with 2, 4, 8 or 32 mg hydrocortisone (Hy)/kg b.w./day, respectively. Animals from the group 4 were injected with decreasing Hy doses (16, 8, 4 and 2 mg Hy/kg b.w./day), changing the dose every week. All Hy-treated groups were compared with the corresponding control animals. Animals were sacrificed 24 h after the last Hy injection. The results obtained by stereological analysis showed differences in the adrenal cortex reaction to Hy application which were dependent on whether physiological (2 and 4 mg) or pharmacological (8; 16, 8, 4 and 2; 32 mg) doses of this glucocorticoid hormone were used. Physiological Hy doses led to a significant increase of the absolute volume of the adrenal cortex, as well as that of zona fasciculata volume and total number of the cells within this zone in comparison with the controls. At the same time, the aplication of pharmacological doses of Hy resulted in a conspicuous decrease of the same parameters comparing to the controls.

Keywords : adrenal gland cortex, hydrocortisone, male rats, stereology

To cite this article

Verica Milošević, Mirjana Hristić, Dobrila Kalafatić, Vesna Koko & Gordana Ušćebrka, «Stereological analysis of the adrenal gland cortex in the male rats treated with hydrocortisone», Acta Stereologica [En ligne], Volume 10 (1991), Number 2 - Stermat '90 (part II) - Dec. 1991, 213-221 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=2005.

About: Verica Milošević

Institute for Biological Research « Siniša Stanković », Department for Cytology and Embryology, 29 Novembra 149, 11060 Beograd, Serbia

About: Mirjana Hristić

Institute for Biological Research « Siniša Stanković », Department for Cytology and Embryology, 29 Novembra 149, 11060 Beograd, Serbia

About: Dobrila Kalafatić

Institute for Biological Research « Siniša Stanković », Department for Cytology and Embryology, 29 Novembra 149, 11060 Beograd, Serbia

About: Vesna Koko

Institute of Medical Research, 11001 Beograd, Serbia

About: Gordana Ušćebrka

Agricultural Faculty, 21000 Novi Sad, Serbia