Acta Stereologica Acta Stereologica -  Volume 12 (1993)  Number 2 - Proceedings of the sixth European congress for stereology - Part one - Dec. 1993 

The effect of improper realignment of serial sections on surface area measurements of 3D-reconstructed objects

Ute Hahn
TU Bergakademie Freiberg, Institut für Stochastik, D-09596 Freiberg, Germany

Abstract

Stereological estimation of surface area of three-dimensional objects using serial sections usually requires perfect realignment of the sections. In physical sectioning often fiducial markers are used to realign the sections. Nevertheless shrinking and other deformations of the slices frequently make it impossible to obtain a precise reconstruction of the spatial arrangement. This inaccuracy causes an error in surface area estimation using methods as e.g. the spatial grid. As shown in the paper, it leads to an overestimation of the surface area. The error is related to the deviation of the “true” slice positions and a simple method is suggested to calculate an upper bound of the error by comparing the relative positions of the fiducial marker profiles on the sections. This method is successfully applied to the estimation of a fish brain’s surface area.

Keywords : 3D-reconstruction, section realignment, spatial grid, surface area

To cite this article

Ute Hahn, «The effect of improper realignment of serial sections on surface area measurements of 3D-reconstructed objects», Acta Stereologica [En ligne], Volume 12 (1993), Number 2 - Proceedings of the sixth European congress for stereology - Part one - Dec. 1993, 169-174 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=1519.