Acta Stereologica

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Eric Masson, Paulette Herlin, Isabelle Galle, Françoise Duigou, Philippe Belhomme, Daniel Bloyet & Anne-Marie Mandard

Automatic classification of cellular elements of solid tumors: application to DNA quantitation

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Abstract

Image Cytometry (ICM) leads often to more reliable DNA measurement of archival solid tumors than Flow Cytometry (FCM) due to sorting of unwanted nuclear elements (inflammatory and stromal cell nuclei, nuclear debris). Nevertheless, interactive sorting is time consuming and unable to give statistically valuable results in an acceptable delay for clinical oncology. The strategy, first results and output of an automatic cell classifier developed within the framework of the optimization of a DNA analyzer are here presented.

Keywords : automatic cell classifier, DNA cytometry, image analyzer, solid tumors

Pour citer cet article

Eric Masson, Paulette Herlin, Isabelle Galle, Françoise Duigou, Philippe Belhomme, Daniel Bloyet & Anne-Marie Mandard, «Automatic classification of cellular elements of solid tumors: application to DNA quantitation», Acta Stereologica [En ligne], Volume 13 (1994), Number 1 - Proceedings of the sixth European congress for stereology - Part two - May 1994, 75-81 URL : https://popups.ulg.ac.be/0351-580x/index.php?id=1021.

A propos de : Eric Masson

LEI/ISMRA F. 14050 Caen Cedex France; lnformatic Department - F. Baclesse Center F. 14021 Caen France

A propos de : Paulette Herlin

Pathology Department - F. Baclesse Center F. 14021 Caen France

A propos de : Isabelle Galle

Pathology Department - F. Baclesse Center F. 14021 Caen France

A propos de : Françoise Duigou

Pathology Department - F. Baclesse Center F. 14021 Caen France

A propos de : Philippe Belhomme

LEI/ISMRA F. 14050 Caen Cedex France

A propos de : Daniel Bloyet

LEI/ISMRA F. 14050 Caen Cedex France

A propos de : Anne-Marie Mandard

Pathology Department - F. Baclesse Center F. 14021 Caen France